Practicals · BIOC

Biochemistry

105 practical items from the NMC curriculum and the NBEMS DNB curriculum, grouped by the curriculum's own sections.

Links point to videos and pages published by others; SolvedPGSS does not host them. Check any technique against your department's protocol before you use it on a patient.

105 items

General Lab Skills & Equipment

Qualitative Practicals (UG-level)

Qualitative tests for carbohydrates

Lab techniqueIndependently (NMC)

Qualitative tests for lipids

Lab techniqueIndependently (NMC)

Qualitative analysis of normal and abnormal urine constituents

Lab techniqueIndependently (NMC)

Paper chromatography for amino acid separation

Lab techniqueIndependently (NMC)

Enzyme & Standard Curve Techniques

Clinical Analyte Estimation

Blood glucose estimation

Lab techniqueIndependently (NMC)

Serum electrolyte estimation

Lab techniqueIndependently (NMC)

Arterial Blood Gas (ABG) analysis

InterpretationIndependently (NMC)

Urinary microalbumin estimation

InterpretationIndependently (NMC)

Cardiac biomarker estimation (CK, CK-MB, troponin, LDH)

InterpretationIndependently (NMC)

Point-of-care testing (POCT)

InstrumentIndependently (NMC)

Sepsis marker estimation

Interpretation

Plasma ammonia estimation

Lab technique

Serum myoglobin estimation

Lab technique

Apolipoprotein A and B (Apo A, Apo B) estimation

Lab technique

Acid phosphatase estimation

Lab technique

Electrophoresis & Protein Separation

Serum protein electrophoresis

Lab techniqueIndependently (NMC)

Lipoprotein electrophoresis

Lab techniqueIndependently (NMC)

Hemoglobin (Hb) electrophoresis

Lab techniqueIndependently (NMC)

Western blotting technique

Lab technique

Immunofixation electrophoresis

Lab technique

Specialized Estimations & Body Fluid Analysis

ELISA (Enzyme-Linked Immunosorbent Assay) technique

Lab technique

Radioimmunoassay (RIA) technique

Lab technique

Radioactivity measurement technique

Lab technique

Clinical Laboratory Quality Management

Biomedical waste management (laboratory)

Communication / admin

Molecular Biology Techniques

RNA isolation technique

Lab techniqueIndependently (NMC)

cDNA synthesis by reverse transcription

Lab techniqueIndependently (NMC)

PCR (conventional)

Lab techniqueIndependently (NMC)

Real-time PCR

Lab techniqueIndependently (NMC)

Reverse transcriptase PCR (RT-PCR)

Lab techniqueIndependently (NMC)

Primer designing

Lab techniqueIndependently (NMC)

Blotting techniques (Southern/Northern/Western)

Lab techniqueIndependently (NMC)

Advanced/Supervised Techniques

Ultracentrifugation

InstrumentUnder supervision (NMC, at least 5 of list)

Plasmid isolation

Lab techniqueUnder supervision (NMC, at least 5 of list)

Cell culture technique and facility setup

Lab techniqueUnder supervision (NMC, at least 5 of list)

High-Performance Liquid Chromatography (HPLC)

InstrumentUnder supervision (NMC, at least 5 of list)

Liquid Chromatography-Mass Spectrometry (LC-MS)

InstrumentUnder supervision (NMC, at least 5 of list)

Fluorescent In-Situ Hybridization (FISH)

Lab techniqueUnder supervision (NMC, at least 5 of list)

DNA fingerprinting

Lab techniqueUnder supervision (NMC, at least 5 of list)

Immuno-electrophoresis

Lab techniqueUnder supervision (NMC, at least 5 of list)

Therapeutic Drug Monitoring (TDM)

InterpretationUnder supervision (NMC, at least 5 of list)

Flow cytometry

Lab techniqueUnder supervision (NMC, at least 5 of list)

Nephelometry

InstrumentUnder supervision (NMC, at least 5 of list)

HLA typing

Lab techniqueUnder supervision (NMC, at least 5 of list)

Analytical Instrumentation

Colorimetry

Instrument

Spectrophotometry

Instrument

Atomic Absorption Spectrophotometry (AAS)

Instrument

Flame photometry

Instrument

Fluorometry

Instrument

Turbidimetry

Instrument

Biosensors (chemical sensors)

Instrument

Osmometry

Instrument

Reflectance photometry

Instrument

Nuclear Magnetic Resonance (NMR) spectroscopy

Instrument